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Journal: Cell Reports Medicine
Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration
doi: 10.1016/j.xcrm.2026.102937
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software
Journal: Nature Communications
Article Title: Defective O-fucosylation of EDIL3 attenuates invasion and vascular remodeling of trophoblasts via LIFR signaling pathway in preeclampsia
doi: 10.1038/s41467-026-73139-4
Figure Lengend Snippet: A Representative images of placental villous explants cultured in rEDIL3 WT and rEDIL3 T88A groups. The bar represents 50 μm. B Western blot analysis of STAT3 and p-STAT3 expression, as well as the expression of EMT markers (E-cadherin, N-cadherin, Vimentin and Snail). C Transwell assay analysis of cell invasion and migration ability of HTR8/SVneo cells. D Bright field images of tube formation. E Western blot analysis of angiogenesis-related protein (CD31, CD34, VE-cadherin, VEGFR2 and VEGFA) expression. F CAM assay analysis of vascular formation. Data ( A , C , D , F ) are presented as mean ± SEM (3 independent biological replicates). Two-tailed unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file.
Article Snippet: The membranes were incubated with blocking buffer containing 5% BSA (CAS:9048-46-8, Solarbio, China) and probed with the following primary antibodies at 4 °C overnight: poFUT1 (Cat No.14929-1-AP, Proteintech, 1:1000), EDIL3 (Cat No.12580-1-AP, Proteintech, 1:1000), His (Cat No.66005-1-Ig, Proteintech, 1:1000), CD31 (Cat No.11265-1-AP, Proteintech, 1:1000), CD34 (Cat No.BS45128, Bioworld, 1:1000), E-cadherin (Cat No.20874-1-AP, Proteintech, 1:1000), N-cadherin (Cat No.22018-1-AP, Proteintech, 1:1000), Vimentin (Cat No.10366-1-AP, Proteintech, 1:2000), Snail (Cat No.13099-1-AP, Proteintech, 1:1000), VE-cadherin (Cat No.sc-515467, SantaCruz, 1:500), VEGFR2(Cat No. 26415-1-AP, Proteintech, 1:1000), VEGFA (Cat No.sc-7269, SantaCruz, 1:500), PCNA (Cat No.AF0261, Beyotime, 1:1000), Cyclin D1 (Cat No.AF0126, Beyotime, 1:1000), Cyclin E1 (Cat No.AF6384, Beyotime, 1:1000), SQSTM1 (Cat No.18420-1-AP, Proteintech, 1:1000), LAMP2A (Cat No.66301-1-Ig, Proteintech, 1:1000), HSPA8 (Cat No.66442-1-Ig, Proteintech, 1:1000), ACTB (Cat No.20536-1-AP, Proteintech, 1:3000),
Techniques: Cell Culture, Western Blot, Expressing, Transwell Assay, Migration, Chick Chorioallantoic Membrane Assay, Two Tailed Test